SML2948

Mca-APK(Dnp)-OH trifluoroacetate

≥97% (HPLC)

• Synonym(s): Mca-APK(Dnp) TFA salt (Mca = 7-Methoxycoumarin-4-yl; Dnp = 2,4-dinitrophenyl), Mca-Ala-Pro-Lys(Dnp) TFA salt, Mca-Ala-Pro-Lys(Dnp)-OH TFA salt
• Empirical Formula (Hill Notation): C₃₂H₃₆N₆O₁₂ · xC₂HF₃O₂
• Molecular Weight: 696.66 (free base basis)
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: film or powder
• storage condition: desiccated
• color: white to dark beige
• storage temp.: −20°C

Description

Biochem/physiol Actions

Mca-APK(Dnp)-OH is a widely used fluorescent substrate for angiotensin-converting enzyme 2 (ACE2 cleaves between P & K). Proteolytic cleavage separates 7-methoxycoumarin-4-acetyl (Mca) from the fluorescence-quenching group 2,4-dinitrophenyl (Dnp), resulting in increased Mca fluorescence (Ex: 320-328 nm, Em: 405-420 nm). The fluorescence increase over background upon Mca-APK(Dnp) cleavage is more than 14-fold higher than that of Mca-YVADAPK(Dnp), while only Mca-YVADAPK(Dnp), but not Mca-APK(Dnp), can also be used for assessing caspase-1 (ICE) activity.

Popular fluorescent substrate for angiotensin-converting enzyme 2 (ACE2).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable