SRP0173
Jak3 Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym(s):
JAK3, Janus kinase 3, Just another kinase 3, LJAK, Leukocyte Janus kinase
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
General description
Human Jak3 (GenBank Accession No. NM_000215), (amino acids 781-end) with N-terminal His tag, MW = 37 kDa, expressed in Baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 1 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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