T9784
Tricine
BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)
Synonym(s):
N-[Tris(hydroxymethyl)methyl]glycine
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About This Item
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
Beilstein:
1937804
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
product line
BioXtra
Quality Level
Assay
≥99% (titration)
form
powder
impurities
≤0.005% Phosphorus (P)
≤0.1% Insoluble matter
ign. residue
≤0.1%
pH
4.0-6.0 (1 M in H2O)
useful pH range
7.4-8.8
pKa (25 °C)
8.1
solubility
H2O: 1 M, clear, colorless
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General description
The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.
Application
Buffer component for separation of low molecular weight peptides.
Tricine has been used:
- in a polymerase chain reaction (PCR) buffer used in the single-cell collection
- as a component of lactotroph feeding medium for culturing lactotrophs
- to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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