ZRB1494
Anti-p-Ubiquitin (pSer65) Antibody, clone 2N21 ZooMAb® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells
Synonym(s):
Ub
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About This Item
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2N21, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
species reactivity
human
1 of 4
This Item | ZRB1224 | ZRB1033 | ZRB1371 |
---|---|---|---|
conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
species reactivity human | species reactivity human | species reactivity human | species reactivity human |
clone 2N21, monoclonal, recombinant monoclonal | clone 2G10, recombinant monoclonal | clone 3C10, recombinant monoclonal | clone 5B9, monoclonal, recombinant monoclonal |
antibody form purified antibody | antibody form purified antibody | antibody form purified antibody | antibody form purified antibody |
biological source rabbit (recombinant) | biological source rabbit (recombinant) | biological source rabbit (recombinant) | biological source rabbit (recombinant) |
storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Immunogen
Application
Flow Cytometry Analysis: 0.1 μg from a representative lot detected p-Ubiquitin (pSer65) in one million PC-3 cells treated with CCCP.
Peptide Inhibition Assay: Target band detection in lysate from HeLa cells transfected with PINK and treated with CCCP (15 mM, 5 h) was prevented by preblocking of a representative lot with the phosphorylated Ser 65 ubiquitin peptide, but not by corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-Ubiquitin (pSer65) in HeLa cells transfected with PINK and treated wtih CCCP.
Affinity Binding Assay: A representative lot of this antibody bound phosphorylated serine 65 Ubiquitin peptide with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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