794-0036
HPLC Analysis of pBr322 DNA-Hae III Digest on TSKgel® DEAE-NPR
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: TSKgel DEAE-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (with 5 mm DEAE-NPR guard column) (813075)
mobile phase: [A] 1 M sodium chloride/25 mm Tris-HCl, pH 9.0; [B] 25 mM Tris-HCl, pH 9.0
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 260 nm
injection: 20 μL of 50 μg/mL Hae III digest of pBR322 DNA
suitability
application for HPLC
application(s)
life science and biopharma
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