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G005962

HPLC Separation of an Oligonucleotide and Its Degradation Fragments on Proteomix® SAX-NP5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)
mobile phase: (A) 25 mM Tris, 1 mM EDTA 10% acetonitrile (8.0 pH); (B) A + 1.0 M NaCl
gradient: 0 to 75% B in 50 min
flow rate: 0.5 mL/min
detector:
sample: Oligonucleotide (MW 12196) and its degradation fragments

suitability

application for HPLC

application(s)

forensics and toxicology
life science and biopharma

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