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Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ABSTRACT

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Acetone, LR, ≥99%

Sigma-Aldrich

Acetone, AR, ≥99.5%

Sigma-Aldrich

Hydrochloric acid solution, 0.1 N

Sigma-Aldrich

Hydrochloric acid, LR, 35-38%

Sigma-Aldrich

Aluminum oxide, mesoporous, MSU-X (wormhole), average pore size 3.8 nm

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥99%

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Acetone, ≥99.5%, ACS reagent

Sigma-Aldrich

Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥98.5% (HPLC), from Saccharomyces cerevisiae

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥99% (HPLC), from starch

Sigma-Aldrich

D-(+)-Trehalose dihydrate, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥99% (HPLC), from Saccharomyces cerevisiae

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Hydrochloric acid solution, ~6 M in H₂O, for amino acid analysis

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

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Hydrochloric acid solution, 32 wt. % in H₂O, FCC

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Hydrochloric acid, 36.5-38.0%, BioReagent, Molecular Biology

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Lipase acrylic resin, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

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Methanol, LR, ≥99%

Sigma-Aldrich

Methanol, AR, ≥99.5%

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

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Methanol, low water for titration

Sigma-Aldrich

Methanol, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Methanol, anhydrous, 99.8%