HomeCustom DNA Oligos Modifications
Custom DNA Oligos Modifications
We offer over 200 modifications for both DNA and RNA oligonucleotides and probes. We can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.
In addition to standard fluorescent modifications, we have the capabilities to provide dual-labeled probes including Molecular Beacons, LightCycler® Probes, and Scorpions® Probes.
Modification Categories
| Fluorescent | Positions | Excitation Maximum (nm) | Emission Maximum (nm) | Fluorescent Color |
|---|---|---|---|---|
| 6-FAM™ (fluorescein) | 5' End, 3' End | 495 | 520 | Green |
| ROX™ | 5' End, 3' End | 586 | 610 | Orange |
| Cyanine 3 | 5' End, 3' End | 549 | 566 | Green-Yellow |
| Cyanine 5 | 5' End, 3' End | 646 | 669 | Red |
| Cyanine 5.5 | 5' End | 675 | 694 | Red |
| 6-FAM dT | 5' End, Internal | 495 | 520 | Green |
| HEX™ | 5' End | 535 | 556 | Green |
| JOE™ | 5' End, 3' End | 529 | 555 | Green |
| 6-Carboxy-rhodamine 6G™ | 5' End, 3' End | 524 | 550 | Green |
| TAMRA | 3' End | 557 | 583 | Green-Yellow |
| TAMRA NHS Ester | 5' End | 557 | 583 | Yellow |
| TET™ | 5' End | 521 | 536 | Green |
| TxRd (Sulforhodamine 101-X) | 5' End, 3' End | 597 | 616 | Red-Orange |
| A488 (Sulfonated Fluorescein 488) | 5' End, 3' End | 495 | 519 | Green |
| WellRED D2-PA | 5' End | 750 | 770 | Red-Infrared |
| WellRED D3-PA | 5' End | 685 | 706 | Red |
| WellRED D4-PA | 5' End | 650 | 670 | Red |
| Attachment | Positions | Purifications | ||
| Amine | ||||
| DMS(O)MT-Amino-Modifier-C6 | 5' End | Cartridge, HPLC | ||
| Amino-Modifier-C3-TFA | 5' End | Desalt, HPLC | ||
| Amino-Modifier-C12 | 5' End | Cartridge, HPLC | ||
| Amino-Modifier-C6-TFA | 5' End | Desalt, PAGE | ||
| Amino-dT | 5' End | Cartridge, HPLC | ||
| Amino-Modifier-5 | 5' End | Cartridge, HPLC | ||
| Amino-Modifier-C2-dT | Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Amino-Modifier-C6-dT | Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 3'-Amino-Modifier-C7 | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Glycerol | ||||
| Glyceryl | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Phosphate | ||||
| Phosphate | 3', 5' End | Desalt, PAGE (5') Desalt, Cartridge, HPLC, PAGE (3') | ||
| Phosphate II | 5' End | Cartridge, HPLC | ||
| Thiol | ||||
| 5'-Thiol-Modifier C6 S-S | 5' End, Internal | Cartridge, HPLC | ||
| 3'-Thiol-Modifier-C3 S-S | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Binding | Positions | Purifications | ||
| Biotin | ||||
| 5'-Biotin | 5' End | Desalt, Cartridge, HPLC, PAGE | ||
| Biotin | Internal | HPLC, PAGE | ||
| Biotin-dT | 5' End, Internal | HPLC, PAGE | ||
| Biotin-TEG | 5' End, Internal | Cartridge, HPLC | ||
| 3'-Biotin-TEG | 3' End | HPLC, PAGE | ||
| Digoxigenin | ||||
| Digoxigenin | 5' End, 3' End | HPLC | ||
| Dinitrophenol | ||||
| 2,4-Dinitrophenyl-TEG | 5' End, Internal | HPLC | ||
| Spacer | Positions | Purifications | ||
| Spacer | ||||
| Spacer 9 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Spacer 12 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Spacer 18 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Spacer C3 | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 3'-Spacer-C3 | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Analog | Positions | Purifications | ||
| Chain Terminating | ||||
| 3'-dA | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 3'-dG | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 3'-dC | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 3'-dT | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Chemotherapeutic | ||||
| Cytosine Arabinoside (araC) | Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 2',3'-dideoxycytidine (ddC) | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Contamination Prevention | ||||
| Ribo U | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Crosslinking | ||||
| 8-Br-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 8-Br-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-Br-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-Br-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-F-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-I-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-I-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 2-Thio-dT | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 4-Thio-dT | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 6-Thio-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Damage and Repair | ||||
| 2'-Deoxypseudouridine | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5,6-Dihydro-dT | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5,6-Dihydro-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-OH-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-OH-dU | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 8-Oxo-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 8-Oxo-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Thymidine Glycol | 5' End, Internal | PAGE | ||
| TMP-F-dU, F-dC | Internal | Desalt, Cartridge, HPLC, PAGE | ||
| dUracil | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Degenerate | ||||
| 2'-Deoxynebularine | 5' End, Internal | Cartridge, HPLC, PAGE | ||
| Derivative K (dK) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Derivative P (dP) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Inosine | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 5-Nitroindole | 5' End, Internal, 5' End | Desalt, Cartridge, HPLC, PAGE | ||
| 3-Nitropyrrole | 5' End, Internal, 3' End | Desalt (Internal only), Cartridge, HPLC, PAGE | ||
| Duplex Stabilizing | ||||
| 2,6-Diaminopurine | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 5-Me-dC | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Fluorescent | ||||
| 2-Aminopurine | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Etheno-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Hybridization Specificity | ||||
| *Locked Nucleic Acid | 5' End, Internal, 3' End | Cartridge, HPLC, PAGE | ||
| Methylation Mutagenesis | ||||
| N6-Me-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| O6-Me-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| O4-Me-dT | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| No Base | ||||
| dSpacer | Internal | Desalt, Cartridge, HPLC, PAGE | ||
| siRNA Guide Strand Selection | ||||
| 5'-OMe-dT | 5' End | Desalt, HPLC, PAGE | ||
| Structure and Function | ||||
| 7-Deaza-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 7-Deaza-dG | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 7-Deaza-dX | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| 7-Deaza-8-Aza-dA | 5' End, Internal | Desalt, Cartridge, HPLC, PAGE | ||
| dA-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| dG-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| dC-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| dT-5' (Inverse Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Translation Termination | ||||
| Puromycin | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Intercalation | Positions | Purifications | ||
| Acridine | ||||
| Acridine | Internal | Desalt, Cartridge, HPLC, PAGE | ||
| Psoralen | ||||
| C2 | 5' End | Desalt, Cartridge, HPLC, PAGE | ||
| C6 | 5' End | Desalt, Cartridge, HPLC, PAGE | ||
| Antisense | Positions | Purifications | ||
| Cholesterol | ||||
| Cholesteryl-TEG | 5' End, Internal | Cartridge, HPLC | ||
| 3'-Cholesteryl-TEG | 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Methyl RNA | ||||
| 2'-OMe-RNA A | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 2'-OMe-RNA G | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 2'-OMe-RNA C | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| 2'-OMe-RNA U | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| Thiophosphate | ||||
| Thiophosphate (S-Oligo) | 5' End, Internal, 3' End | Desalt, Cartridge (5' End, Internal) Desalt, Cartridge, HPLC, PAGE (3' End) | ||
| 2' → 5' Synthesis | ||||
| dA-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| dG-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| dC-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
| dT-5' | 5' End, Internal, 3' End | Desalt, Cartridge, HPLC, PAGE | ||
Phosphorothioates (or S-oligos)
Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.
Minimum Yield Guarantees | ||||
|---|---|---|---|---|
| Modified Oligos (O.D./µg)* | ||||
| Scale (µmole) | Desalt | Cartridge | HPLC | PAGE |
| 0.05 | 2/60 | 0.4/12 | 0.4/12 | 0.2/6 |
| 0.2 | 5/150 | 1/30 | 1/30 | 0.4/12 |
| 1.0 | 16/480 | 5/150 | 5/150 | 2/60 |
| 10 | 160/4,800 | NA | 52/1,560 | NA |
| 15 | 240/7,200 | NA | 76/2,280 | NA |
*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.
| Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )* | ||||
|---|---|---|---|---|
| Scale (µmole) | Desalt | Cartridge | HPLC | PAGE |
| 0.05 | 3.5/105 | 0.7/21 | 0.7/21 | 0.35/10.5 |
| 0.2 | 8.4/252 | 2.1/63 | 1.75/52.5 | 0.7/21 |
| 1.0 | 28/840 | 8.4/252 | 9.1/273 | 3.5/105 |
| 10 | 280/8,400 | NA | 91/2,730 | NA |
| 15 | 420/12,600 | NA | 133/3,990 | NA |
Shipping Schedule | ||||
|---|---|---|---|---|
| Purification | Desalt | Cartridge | HPLC | PAGE |
| Standard Modifications | 5-7 days or less | 5-7 days or less | 5-7 days or less | Inquire |
| Post-Synthesis Modifications | 7-10 days | 7-10 days | 7-10 days | Inquire |
*For complete label license information, please visit sigmaaldrich.com/oligolicenses.
Materials
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