Cold Hard Facts: Implementing High Cell Density Cryopreservation in Your Intensified Seed Train

WEBINAR

This presentation explores the use of a high cell density cryopreservation (HCDC) method that utilizes bag assemblies specifically designed for freezing cells at higher densities and larger volumes, enabling them to directly inoculate the N-2 bioreactor. The integration of a perfused N-1 step in the upstream process is highlighted to either decrease facility footprint or to inoculate final fed-batch N-stage with a higher cell density than a conventional fed-batch. This streamlined method of initiating cell expansion can reduce the number of intermediate cell culture unit operations, allowing for a shortened expansion process by 10 days and to decrease the runtime of the final N-stage bioreactor.

In this webinar, you will learn about:

• Receiving an overview of intensified upstream processing
• How the HCDC process employs a closed single-use system, significantly reducing contamination risk and improving reproducibility
• How using a perfused N-1 step can either decrease your facility footprint or increase your biomass generation in your seed train