C6079

Collagenase + protease inhibitor

Name: Collagenase + protease inhibitor
Brand: SIGMA

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

Synonim(y):

Preparat kolagenazy, Roztwór inhibitora proteazy

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About This Item

Numer WE:

3.4.24.3 (BRENDA, IUBMB)

Kod UNSPSC:

12352204

NACRES:

NA.54

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Pomoc techniczna

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Pomoc techniczna

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Pozwól nam pomóc

pochodzenie biologiczne

bacterial (Clostridium histolyticum)

Poziom jakości

200

Formularz

solid

aktywność właściwa

≥800 CDU/mg solid
2-5 FALGPA units/mg solid

masa cząsteczkowa

68-130 kDa

rozpuszczalność

water: soluble

Warunki transportu

dry ice

temp. przechowywania

−20°C

informacje o genach

rat ... LOC296948(296948)

Zastosowanie

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

Działania biochem./fizjol.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca²⁺) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn²⁺ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Inne uwagi

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.