G2N10

GenElute^™ Plant Genomic DNA Miniprep Kit

sufficient for 10 purifications

• Synonim(y): Plant Genomic DNA Miniprep, Gen Elute
• Kod UNSPSC: 41105501
• NACRES: NA.55

Właściwości

• zastosowanie: sufficient for 10 purifications
• Poziom jakości: 200
• charakterystyka ekologicznej alternatywy: Inherently Safer Chemistry for Accident Prevention; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• kategoria ekologicznej alternatywy: Aligned,
• temp. przechowywania: 15-25°C

Opis

Opis ogólny

The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.

The GenElute Plant Genomic DNA Purification Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of plant species. This kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.

Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.

The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Zastosowanie

904 Plant Genomic DNA Miniprep Kit:

• w izolacji arbuskularnego DNA zarodników grzybów mikoryzowych (AM)
• w oczyszczaniu genomowego DNA ekstrahowanego bromkiem cetylotrimetyloamoniowym (CTAB)
• w izolacji genomowego DNA z liści

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:

• PCR
• restriction endonuclease digestion
• cloning
• Southern blots

Cechy i korzyści

• Starting material: Up to 100 mg of plant tissue
• Expected yield: Up to 20 μg
• Elution volume: 100 - 200 μl
• Time required: < 40 min
• RNase treatment required: No

Zasada

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Elementy zestawu są też dostępne oddzielnie

• C2112 Column Preparation Solution

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H302 + H332,H314,H412
• Zwroty wskazujące środki ostrożności: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Zagrożenia dodatkowe: EUH032
• Kod klasy składowania: 8A - Combustible corrosive hazardous materials
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable