P5542

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

• Synonim(y): 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
• CAS Number: 37288-19-0
• Numer WE: 4.6.1.13 (BRENDA, IUBMB)
• Numer WE: 232-638-2
• Numer MDL: MFCD00131896
• Kod UNSPSC: 12352204
• NACRES: NA.32

Właściwości

• Formularz: lyophilized powder
• Poziom jakości: 300
• aktywność właściwa: ≥1,000 units/mg protein (Lowry)
• obecność zanieczyszczeń: Phopholipase C (lecithinase) ≤1 units/mg protein; Sphingomyelinase ≤40 units/mg protein
• temp. przechowywania: −20°C
• informacje o genach: Bacillus cereus E33L ... BCZK3513(3026815)

Opis

Opis ogólny

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Zastosowanie

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Działania biochem./fizjol.

Used for the release of GPI anchored proteins from the membrane.

Definicja jednostki

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Postać fizyczna

Contains phosphate buffer salts, EDTA and stabilizer

Komentarz do analizy

Acetylcholinesterase is measured according to Ellman, et al.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)