P9290

Proteinase K–Agarose from Tritirachium album

lyophilized powder

• Synonim(y): Proteinase K-Agarose
• Numer MDL: MFCD00132131
• Kod UNSPSC: 41106500
• NACRES: NA.56

Właściwości

• pochodzenie biologiczne: microbial (T.album; T. ALBUM)
• Poziom jakości: 200
• Formularz: lyophilized powder
• zakres etykietowania: 30-140 units per g solid
• metody: DNA extraction: suitable; RNA extraction: suitable; protein extraction: suitable
• pęcznienie: 1 g swells to ~5 mL
• temp. przechowywania: −20°C

Opis

Zastosowanie

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

Działania biochem./fizjol.

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

Cechy i korzyści

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

Definicja jednostki

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Postać fizyczna

Lyophilized powder stabilized with lactose

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable