NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• Synonim(y): Zestaw do szybkiej izolacji jąder komórkowych ssaków
• Kod UNSPSC: 12352207
• NACRES: NA.32

Właściwości

• zastosowanie: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Poziom jakości: 200
• okres trwałości: 1 yr at 2‑8 °C
• opakowanie: pkg of 1 kit
• Zastosowanie: cell analysis
• obecność zanieczyszczeń: nuclease and protease, free
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Zastosowanie

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Działania biochem./fizjol.

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Inne uwagi

All procedures should be carried out on ice or 2-8°C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable