NUC201

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)

• Synonim(y): Izolacja jąder metodą wirowania sacharozy
• Kod UNSPSC: 12352207
• NACRES: NA.32

Właściwości

• zastosowanie: sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)
• Poziom jakości: 200
• opakowanie: pkg of 1 kit
• warunki przechowywania: dry at room temperature
• Zastosowanie: cell analysis
• obecność zanieczyszczeń: nuclease and protease, free
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Działania biochem./fizjol.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

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Tylko elementy zestawu

• Nuclei PURE Lysis Buffer 180 mL

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS09
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H318,H410
• Zwroty wskazujące środki ostrożności: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Klasyfikacja zagrożeń: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable