Sign Into View Organizational & Contract Pricing
About This Item
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
Beilstein:
637031
EC Number:
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.22
Quality Level
Assay
96% (NT)
form
liquid
color
APHA: ≤20
refractive index
n20/D 1.398 (lit.)
n20/D 1.398
bp
93-94 °C/18 mmHg (lit.)
solubility
95% ethanol: 4.5 g/10 mL, clear, colorless
density
1.121 g/mL at 20 °C
1.101 g/mL at 25 °C (lit.)
functional group
carbonate
shipped in
wet ice
storage temp.
2-8°C
SMILES string
CCOC(=O)OC(=O)OCC
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.
Application
Diethyl pyrocarbonate may be used in the following studies:
- For the modification of histidyl residues in proteins.
- As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
- For the modification of linear and supercoiled plasmid DNAs.
- As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
- For cabethoxylation of histidine residues of actin.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
156.2 °F - closed cup
Flash Point(C)
69 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service