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Immobilization of antibodies for use in ultrasensitive electrochemical immunosensors using the abundant amine groups and a large electroconductive surface area of reduced graphene oxide-tetraethylene pentamine (rGO-TEPA).
Amount of NH₂ groups: In order to estimate the amount of NH₂ groups in rGO-TEPA a reaction with tetrabromophthalic anhydride was performed and the Br amount was quantified by the Schöniger flask test. The value obtained corresponds to 1.1 mmol NH₂/g.