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About This Item
Linear Formula:
K2HPO4 · 3H2O
CAS Number:
Molecular Weight:
228.22
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Quality Level
product line
ReagentPlus®
Assay
≥99.0%
form
powder or crystals
pH
9.2-9.4 (25 °C, 50 g/L)
solubility
water: 100 mg/mL, clear, colorless
storage temp.
room temp
SMILES string
O.O.O.[K+].[K+].OP([O-])([O-])=O
InChI
1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2
InChI key
XQGPKZUNMMFTAL-UHFFFAOYSA-L
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General description
Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.
Application
Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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