biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by
affinity chromatography (affinity tag purification)
species reactivity
all
concentration
1 mg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
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General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Method of purification - Affinity tag purification
Specificity
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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