grade
Molecular Biology
Quality Level
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected
storage temp.
room temp
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General description
SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.
Application
SSC Buffer 20× Concentrate has been used
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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