biological source
mouse adipose tissue
packaging
tube of 5 μg 85011425-DNA-5UG
pkg of vial of cells 85011425-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse C3H/An connective tissue
Cell Line Description
Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.
Application
Virus studies: PRV, VSV (Indiana), HSV
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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