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UNSPSC Code:
41106514
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biological source

African green monkey kidney

description

Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

packaging

tube of 5 μg 85020206-DNA-5UG
pkg of vial of cells 85020206-1VL

growth mode

Adherent

karyotype

2n = 60

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Vero cells are derived from the kidney of an African green monkey. These are anchorage-dependent cells that have applications in molecular and cell biology research. Vero E6 cells enable achieving high titers of severe acute respiratory syndrome coronavirus (SARS-CoV virus) and are also used in the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.

Cell Line Origin

Monkey African Green kidney

Cell Line Description

A subclone of Vero76 (Sigma catalog no. 85020205) exhibiting a similar range of virus susceptibility (haemorrhagic viruses). They show some degree of contact inhibition and are suitable for supporting the growth of slowly replicating viruses.

Application

VERO C1008 has been used for transfection, cultivation and propagation of West Nile virus, yellow fever virus, ZIKA, Ebola virus, Sabethes flavivirus (SbFV), Crimean-Congo Haemorrhagic Fever (CCHF), Human-derived Lake Victoria Marburg virus strain Popp.

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

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