biological source
human breast
packaging
tube of 5 μg 92020424-DNA-5UG
pkg of vial of cells 92020424-1VL
growth mode
Adherent
karyotype
Modal no.′s 62 and 64, near triploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian breast adenocarcinoma
Cell Line Description
Isolated from pleural effusions of a breast cancer patient.
Application
MDA-MB-231 has been used to study:
- the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
- the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
- the transendothelial migration rates of the cells under different shear stress levels
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Certificates of Analysis (COA)
Lot/Batch Number
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