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About This Item
Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
Molecular Weight:
216.04 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.25
biological source
synthetic
Quality Level
form
powder
impurities
≤0.1% Inorganic phosphorus
≤1.0 mol % L-α-isomer
mp
102-104 °C (lit.)
solubility
water: 50 mg/mL, clear, colorless
SMILES string
O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
InChI
1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
InChI key
ROPZSVKNEIIIDE-UHFFFAOYSA-L
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Application
β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
β-glycerophosphate is used in homegenization solutions for muscles extracts following fixation.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.
Quality
Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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