Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Hanks′ Balanced Salt solution has been used:

• as a component of the culture medium for culturing of mouse cortical slices
• as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
• for in situ perfusions of mice liver

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.