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About This Item
Empirical Formula (Hill Notation):
C12H6NNaO4
CAS Number:
Molecular Weight:
251.17
Beilstein:
3794971
EC Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75
product line
BioReagent
Quality Level
form
powder
composition
Dye content, ~80%
pH
3.8-6.5, orange to violet
solubility
H2O: soluble
λmax
600 nm
SMILES string
[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
InChI
1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
InChI key
IVGPGQSSDLDOLH-UHFFFAOYSA-M
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Application
For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin sodium salt has been used in viability assay:
- to measure cell viability of A. fumigatus
- to assess cell proliferation
- to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts
Biochem/physiol Actions
Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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