Prestige Antibodies® Powered by Atlas Antibodies

With industry-leading antibody characterization and lot-specific testing, our polyclonal and monoclonal Prestige Antibodies® are designed to detect and target over 80% of the human proteome in immunohistochemistry (IHC), immunofluorescence (IF), Western blot (WB), and other applications. Prestige Antibodies® undergo some of the most stringent validation and characterization available. Prestige Antibodies® are developed using open-access data from the Human Protein Atlas and validated using IHC, WB, ICC-IF, or RNA sequencing and compared to bioinformatic information and literature. Prestige Antibodies® are characterized via tissue microarrays in over 40 different normal human tissues, over 50 cell lines, and over 20 cancer types.
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What Makes Prestige Antibodies® Unique?
All Prestige Antibodies® undergo in-depth validation as well as characterization using the immense amount of supporting data from the Human Protein Atlas project. The low cross reactivity for Prestige Antibodies® to other proteins is the result of careful selection of antigen regions, affinity purification, and are validated and characterized in each application that the antibody has been demonstrated for use. Each antibody is characterized on a lot-to-lot basis with application-specific validation that includes IHC, IF, WB, and or protein array assays.
Enhanced Validation – Another Reason to Trust Prestige Antibodies®
In addition to the standard antibody validation methods to validate and characterize Prestige Antibodies®, we offer enhanced validation which is aligned to the pillars for antibody validation described by the International Working Group for Antibody Validation (IWGAV) for numerous antibodies. Many Prestige Antibodies® undergo one or more enhanced validation methods including genetic strategies, independent antibody verification, orthogonal validation using RNA-seq, and functional assay validation. Learn more about our Enhanced Validation.





IF Analysis
Western Blot
BioInformatics & Literature Search
IHC Analysis
Protein Array
For Research Use Only. Not for use in diagnostic procedures.
Unless otherwise stated in the Product(s) specifications, any Antibody product is sold for internal research use only and may not be used for any other purpose, which includes but is not limited to, any commercial, diagnostic, or therapeutic use. Our validation processes pertain only to research uses and do not confirm or assure that our antibodies can be used for any unauthorized uses as set forth herein.
Related Resources
- Brochure: Prestige Antibodies® Pan-Cancer Markers
Explore our Prestige Antibodies® portfolio classified by tumor type.
- Article: Human Protein Atlas
- Article: Prestige Antibodies® on The Cell Atlas
- Protocol: Immunohistochemistry Procedure
Prestige Antibody Immunohistochemistry Procedure protocol using standardized tissue microarray slides for antibody validation.
- Protocol: Immunofluorescence Procedure
Immunofluorescence Protocol details Prestige Antibodies' subcellular localization studies in A-431, U-2 OS, and U-251MG cell lines.
- Protocol: Prestige Antigens™ Blocking Protocol
Prestige Antigens™ and the corresponding Prestige Polyclonals or Monoclonals are recommended to use according to the standard Prestige Antigen-blocking protocol
- Article: Stem Cell Markers and Antibodies
- Article: Neural Stem Cell Markers and Antibodies
- Article: Mesenchymal Stem Cell Markers and Antibodies
- Brochure: Prestige Antibodies® in Neuroscience
The Human Protein Atlas project has created a complete map of protein expression in all major organs and tissues in the human body.
- Article: Prestige Antibodies® in Breast Cancer Research
- Brochure: Prestige Antibodies® in Colorectal Cancer Research
The Human Protein Atlas project has created a complete map of protein expression in all major organs and tissues in the human body.
- Protocol: Prestige Antibodies® in Western Blot Applications
Sigma-Aldrich.com presents a white paper on Prestige Antibodies® Powered by Atlas Antibodies in Western Blot Application
- Protocol: Prestige Antibodies® in Immunofluorescence Applications
Subcellular protein location often correlates with function; nucleus proteins implicated in gene regulation, mitochondria proteins in energy production.
- Article: Prestige Antibodies® in Organelle Markers
- Prestige Antibodies® Powered by Atlas Antibodies in Immunohistochemistry
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