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MilliporeSigma

Your Guide To
Protein Research

Empower Your Protein Research

Refine your protein research for optimal efficiency and reliability. Explore the potential and significance of proteins using our solutions to extract, isolate, and analyze your proteins of interest. Our diverse portfolio of solutions streamlines the entire process, preserving integrity and protecting against degradation throughout your workflow.

Our reliable methods and innovative detection tools empower you to deliver comprehensive insights into your target proteins' properties and functions.

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Protein Biology Workflow

An Eppendorf 1.5ml tube is in the middle of the image, filled with cell lysate. It is being held by a tube holder that cutes horizontal across the bottom and the middle of the image. A pipette tip at the top center of the image drops two cell lysate drops from the tip into the tube.

Cell Lysis & Extraction

One of the first steps in protein sample prep, cell lysis uses physical or enzymatic methods to break down cells to release its inner components. Consistent and reproducible products for this extraction step are crucial, as the methods used to extract and isolate proteins can often affect the activity, purity, and stability of the protein. Achieve fast and gentle protein extraction with our family of protein extraction reagents:

    The image captures a precise moment in a laboratory setting, where a blue pipette is dispensing a single clear liquid droplet into a small clear container.

    After cell lysis and extraction, the next step is protein purification. Isolating proteins often uses affinity chromatography with antibodies, recombinant or fusion tags, or biotinylated tags. The type of tag and purification system depends on the protein of interest and the expression system. Our portfolio of protein purification tools includes affinity columns and resins, magnetic bead columns, agarose beads and affinity gels for antibody purification, biotinylated agarose bead and affinity gels, and more:

      Antibodies play a crucial role in protein isolation and analysis. As versatile reagents that directly and uniquely bind to a protein or molecule of interest (primary antibodies) or another antibody (secondary antibodies), they enable various techniques to identify the presence (or absence), quantity, and location of their target. We provide highly cited and validated primary, secondary, and recombinant monoclonal antibodies, including ZooMAb® recombinant monoclonal antibodies. Our ZooMAb® recombinant antibodies are proprietary recombinant monoclonal antibodies purified to homogeneity that offer the superior specificity, high reproducibility, and a greener alternative. With our antibodies, analyses such as Western blot, immunohistochemistry, ELISA, and other assays are made easier:

        Protein research workflows are not complete without enzyme tools for protein research, including our collection of highly characterized and high purity proteases, nucleases, and glycosidases. These enzymes are vital for protein isolation, purification, and identification, as well as further proteomics research, including post-translational modifications (PTM) analysis, mass spectrometry (MS) analysis, and tandem MS. Our family of proteins includes:

          An image of a gel stain, showing protein bands. There are nine vertical columns that all have at least three horizontal bands representing the proteins.

          A crucial aspect of the protein research workflow, detecting and quantifying proteins involves measuring the levels of specific proteins in a sample and provides insights into protein expression, abundance, and changes under different experimental conditions. Protein labels and other detection substrates can be used for identification and other assays including enzyme assays, ELISA, imaging, blotting, protein-protein interactions, and more. We also offer a collection of reagents, buffers, and stains for protein electrophoresis and Western blotting:

            Protein Biology Products


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            Extract

            Protect

            • Protease Inhibitor Cocktails

              Protease Inhibitor Cocktails maintain and preserve cellular protein composition following cell lysis. When used with CelLytics, they also preserve target protein activity. These cocktails have been optimized for a wide variety of specific applications, ensuring excellent inhibition and ease of use.

            • Phosphatase Inhibitor Cocktail

              The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

            • ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

              ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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            ZooMAb<sup>®</sup> Antibodies Technology
            ZooMAb® Antibodies Technology
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