It’s not you. It’s your tools.
The right Western choices can take the “ugly” out of your next blot.
Rethink Western blotting with tools and strategies to optimize your Western blots
Our Western blotting tools help tailor your choices to your target.
- Fresh SDS-PAGE gels in 3-minutes with the mPAGE® Lux Casting System
- Publication-ready resolution at a fraction of the cost with mPAGE® precast gels
- Complete selection of PVDF and nitrocellulose membranes to help you make the right choice for transferring your protein of interest
- 30-minute immunodetection with the SNAP i.d.® 2.0 system for cleaner, more consistent data
- Ready-to-use reagents like our ultrasensitive Immobilon® substrates
- Application-specific antibody manufacturing expertise, with over 70,000 tested in Western blot
Show us your ugly blots
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise – except that it will spark creativity in the lab. Simply upload an image (jpg, pdf, or tif format, max file size 10 MB) of your ugliest blot and complete the form below. We’ll also provide tips and tricks for revealing beautiful Western data with a PDF of our latest Protein Blotting Handbook.
Highlighted Electrophoresis and Western Blotting Products
Electrophoresis
Protein gel electrophoresis is a technique used to separate proteins by molecular weight, facilitating analyses such as protein purification, proteomics, and Western Blotting.
mPAGE® Lux Casting System
mPAGE® Gel Casting Kits
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mPAGE® Buffers and Equipment
mPAGE® Protein Molecular Weight Markers
Membrane Transfer
Immobilon® PVDF and nitrocellulose transfer membranes offer high sensitivity and low background for Western blotting and other applications.
Immobilon® NOW Membranes (8.5 cm x 10 m rolls)
mPAGE® Transfer Buffer and Equipment
Immunodetection
Western blotting immunodetection uses antibodies to detect target proteins. After blocking the blotted membranes with transferred proteins, antibodies are incubated, and detection is achieved through chemiluminescence, colorimetric, or fluorescent methods.
Immobilon® Signal Enhancer and Blocking Reagents
Antibodies: Find the right antibody for your application with our Antibody Explorer search tool. Browse and compare antibodies based on clonality, application, species, reactivity, conjugate, host species, and form.
SNAP i.d.® 2.0 Protein Detection Systems for Western Blotting
Immobilon® Chemiluminescence Peroxidase Substrates
Protocols and Resources
Your Western Blot data is only as good as the operator and the resources on hand. Let our team of experts help with the latter. Explore our most popular Western Blot protocols, technical resources, recipe calculators and more!
Western Blot Protocols & Resources
- Protein Blotting Handbook
Download our latest Protein Blotting Handbook for helpful tips, recipes, and protocols.
- Western Blotting Protocol (Immunoblotting Protocol)
Western blot protocol with workflow steps for different blot procedures, describing the electrophoretic transfer of proteins from SDS polyacrylamide gels to sheets of nitrocellulose. Also known as Immunoblotting protcol.
- Cell Lysis and Protein Extraction for Western Blotting
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
- Buffers and Western Blotting Solutions Recipe Calculators
Web Toolbox - science research tools and resources for analytical chemistry, life science, chemical synthesis and materials science.
- Stripping and Reprobing Western Blotting Membranes
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
- Introduction to SDS-PAGE – Separation of Proteins Based on Size
Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.
- 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
- ReadyBlue Protein Gel Staining Protocols
ReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie® stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol.
Antibodies
- Antibody Selector Tool
Explore our vast selection of primary, secondary, and recombinant monoclonal antibodies to buy for ELISA, Western blot, and immunohistochemistry (IHC).
- ZooMAb® Recombinant Antibody Technology
ZooMAb® Recombinant Antibody Technology
- Greener ZooMAb® Antibodies
Greener ZooMAb® antibodies offer sustainable options with low-waste packaging and BSA-free formulation.
- Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
- Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
- Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
- Membrane Immunodetection Devices
Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.
- Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
- Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
- Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.
- Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
- Gel Electrophoresis
Protein gel electrophoresis methods are used for protein separation in preparation for downstream applications like Western blotting and mass spectrometry.
- Western Blotting
Western blotting overview includes protocols and resources for protein detection, extraction, gel electrophoresis, and detection methods.
- Protein Expression
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
- Protein Pull-Down Techniques
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
- Nucleic Acid Gel Electrophoresis
Basics of DNA and RNA gel electrophoresis for separation and purification in various applications.
- DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
- Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
- Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
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