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A1765

Sigma-Aldrich

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥3 units/mg protein

Synonym(s):

Acetate CoA ligase (AMP forming), Acetate thiokinase

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About This Item

CAS Number:
9012-31-1
EC Number:
6.2.1.1 (BRENDA, IUBMB)
EC Number:
232-729-7
MDL number:
MFCD00081267
UNSPSC Code:
12352204
NACRES:
NA.26
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

200

specific activity

≥3 units/mg protein

composition

Protein, 10-30% biuret

storage temp.

−20°C

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This Item
A3352G1270P7173
S-Acetyl-coenzyme A synthetase from baker’s yeast (S. cerevisiae) lyophilized powder, ≥3 units/mg protein

A1765

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae)

Acyl-coenzyme A Synthetase from Pseudomonas sp. ≥2 units/mg protein

A3352

Acyl-coenzyme A Synthetase from Pseudomonas sp.

L-Glutamine Synthetase from Escherichia coli lyophilized powder, 400-2,000 units/mg protein

G1270

L-Glutamine Synthetase from Escherichia coli

Pyruvate Carboxylase from bovine liver buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

P7173

Pyruvate Carboxylase from bovine liver

specific activity

≥3 units/mg protein

specific activity

≥2 units/mg protein

specific activity

400-2,000 units/mg protein

specific activity

5-25 units/mg protein (BCA)

form

lyophilized powder

form

powder

form

lyophilized powder

form

buffered aqueous glycerol solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, 10-30% biuret

composition

-

composition

Protein, ~5% Lowry

composition

-

Application

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae) has been used in the synthesis of adenosine 5′-tetraphosphate and adenosine 5′-pentaphosphate.[1]
S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies.

Biochem/physiol Actions

Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol [2].

Packaging

Package size based on protein content.

Unit Definition

One unit will form 1.0 μmole of S-acetyl coenzyme A from acetate, ATP, and coenzyme A per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing stabilizers as potassium phosphate, sucrose, and reduced glutathione

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCL6723
    SLCG5103
    SLCF8091
    SLBW3883
    108K1548

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    Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase.
    Guranowski A, et al.
    Journal of Bacteriology, 176(10), 2986-2990 (1994)
    Guillaume Baptist et al.
    Nucleic acids research, 41(17), e164-e164 (2013-07-31)
    We have developed a new screening methodology for identifying all genes that control the expression of a target gene through genetic or metabolic interactions. The screen combines mutant libraries with luciferase reporter constructs, whose expression can be monitored in vivo
    Sergio Renilla et al.
    Applied microbiology and biotechnology, 93(5), 2109-2124 (2011-09-02)
    Impairment of acetate production in Escherichia coli is crucial for the performance of many biotechnological processes. Aerobic production of acetate (or acetate overflow) results from changes in the expression of central metabolism genes. Acetyl-CoA synthetase scavenges extracellular acetate in glucose-limited
    Saurabh Sahar et al.
    The Journal of biological chemistry, 289(9), 6091-6097 (2014-01-16)
    The circadian clock regulates a wide range of physiological and metabolic processes, and its disruption leads to metabolic disorders such as diabetes and obesity. Accumulating evidence reveals that the circadian clock regulates levels of metabolites that, in turn, may regulate
    Sara Castaño-Cerezo et al.
    Molecular microbiology, 82(5), 1110-1128 (2011-11-09)
    Lysine acetylation is a well-established post-translational modification widely conserved and distributed in bacteria. Although multiple regulatory roles have been proved, little is known about its regulation. Here, we present evidence that the transcription of the Gcn5-like acetyltransferase YfiQ of Escherichia
    View All Related Papers

    Articles

    Coenzyme A

    Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

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