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M6899

Sigma-Aldrich

Murashige and Skoog Basal Salts with minimal organics

powder, suitable for plant cell culture

Synonym(s):

MSMO

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72
Pricing and availability is not currently available.

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

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assay

99%

assay

-

assay

97%

assay

-

density

1.3 g/mL at 25 °C (lit.)

density

-

density

-

density

-

solubility

H2O: insoluble

solubility

H2O: insoluble <0.04 ppm at 20 °C, toluene: 20 wt. % at 20 °C, methylene chloride: 34 wt. % at 20 °C, chloroform: 35 wt. % at 20 °C

solubility

-

solubility

-

mp

98-101 °C (lit.)

mp

139-143 °C (lit.)

mp

198-202 °C (lit.)

mp

125.5-129.5 °C (lit.)

transition temp

transition temp >270 °C

transition temp

-

transition temp

-

transition temp

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

300

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

With the macro- and micronutrients, and vitamins as described by Linsmaier and Skoog (1965).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M6899 is a variant media) with macro- and micronutrients, vitamins as described by Linsmaier and Skoog.[1]

pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Danger

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 2

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCF4310
    SLCJ7932
    SLCF4311
    SLCC6767
    SLBX2424

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    Organic growth factor requirements of tobacco tissue cultures.
    Linsmaier, E.M. and Skoog, F.
    Physiologia Plantarum, 18, 100-127 (1965)
    Qiaojun Jia et al.
    BMC genomics, 16, 927-927 (2015-11-18)
    sdw1/denso is one of the most important and useful semi-dwarf genes in barley breeding. At least four sdw1/denso alleles have been reported and HvGA20ox 2 is considered as the candidate gene. Up to date, results of studies have not univocally
    Li Wang et al.
    Planta, 249(4), 1107-1118 (2018-12-16)
    In hybrids of Arabidopsis, cotyledons influence the amount and proportion of hybrid vigor in total plant growth. We found Arabidopsis cotyledons are essential for plant growth and in some hybrids for hybrid vigor. In hybrids between C24 and Landsberg erecta
    Okhee Choi et al.
    Plant physiology, 146(2), 657-668 (2007-12-07)
    Pseudomonas fluorescens B16 is a plant growth-promoting rhizobacterium. To determine the factors involved in plant growth promotion by this organism, we mutagenized wild-type strain B16 using OmegaKm elements and isolated one mutant, K818, which is defective in plant growth promotion
    Thelma F Madzima et al.
    Frontiers in plant science, 12, 694289-694289 (2021-09-08)
    Plants respond to abiotic stress stimuli, such as water deprivation, through a hierarchical cascade that includes detection and signaling to mediate transcriptional and physiological changes. The phytohormone abscisic acid (ABA) is well-characterized for its regulatory role in these processes in
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