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SHC002H

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer

Targets no known mammalian genes

Synonym(s):

MISSION®, MISSION® Control Transduction Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51
Pricing and availability is not currently available.

Quality Level

100
200

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

technique(s)

capture ELISA: 109 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

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This Item
SHC004VSHC005VSHC002V
MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer Targets no known mammalian genes

SHC002H

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles shRNA sequence targeting tGFP

SHC004V

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles

MISSION® pLKO.1-puro eGFP shRNA Control Transduction Particles shRNA sequence targeting eGFP

SHC005V

MISSION® pLKO.1-puro eGFP shRNA Control Transduction Particles

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles Targets no known mammalian genes

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles

technique(s)

capture ELISA: 109 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100, 200

General description

This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Components

High titer controls are provided as 10 x 20 μl aliquots.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    06282109MN
    03062077MN

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    Younho Han et al.
    Scientific reports, 6, 35655-35655 (2016-10-19)
    Osterix is a novel bone-related transcription factor involved in osteoblast differentiation, and bone maturation. Because a reciprocal relationship exists between adipocyte and osteoblast differentiation of bone marrow derived mesenchymal stem cells, we hypothesized that Osterix might have a role in
    Regional anatomic and age effects on cell function of human adipose-derived stem cells.
    Bret M Schipper,Kacey G Marra,Wei Zhang,Albert D Donnenberg,J Peter Rubin
    Annals of Plastic Surgery null
    Qi Tang et al.
    International journal of molecular medicine, 40(3), 713-720 (2017-07-22)
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    Yifeng Jin et al.
    Scientific reports, 6, 34661-34661 (2016-10-04)
    Conformational change in helix 12 can alter ligand-induced PPARγ activity; based on this reason, isoquinolinoquinazolinones, structural homologs of berberine, were designed and synthesized as PPARγ antagonists. Computational docking and mutational study indicated that isoquinolinoquinazolinones form hydrogen bonds with the Cys285
    William P Cawthorn et al.
    Bone, 50(2), 477-489 (2011-08-30)
    Wnt10b is an established regulator of mesenchymal stem cell (MSC) fate that inhibits adipogenesis and stimulates osteoblastogenesis, thereby impacting bone mass in vivo. However, downstream mechanisms through which Wnt10b exerts these effects are poorly understood. Moreover, whether other endogenous Wnt
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