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SHC016

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

Synonym(s):

MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

MDL number:
MFCD07785395
UNSPSC Code:
41106609
NACRES:
NA.51
Pricing and availability is not currently available.
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Quality Level

200

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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1 of 4

This Item
SHC216SHC001SHC016H
MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA Targets no known genes from any species

SHC016

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA Targets no known genes from any species

SHC216

MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA Contains no shRNA insert

SHC001

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer Targets no known genes from any species

SHC016H

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

≥1x109 VP/ml (via p24 assay)

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
  • in tumor cells for multicolour imaging[1]
  • in human adult low calcium temperature keratinocytes[2]
  • in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)[3]
  • to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation[4]
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
Pricing

SHC001

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA, Contains no shRNA insert

SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC005

MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA, shRNA sequence targeting eGFP

SHC007

MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA, shRNA sequence targeting luciferase

SHC012

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Plasmid DNA, Contains a gene encoding TagRFP

SHC014

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Zac1 regulates astroglial differentiation of neural stem cells through Socs3
    Schmidt EU, et al.
    Stem Cells, 31(8), 1621-1632 (2013)
    Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes
    Avitabile D, et al.
    The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)
    Brain tumour cells interconnect to a functional and resistant network
    Osswald M, et al.
    Nature, 528(7580), 93-93 (2015)
    Ryusuke Nakajima et al.
    PloS one, 12(6), e0179585-e0179585 (2017-06-29)
    A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA that contains a SINE (short interspersed element)-B1F motif that was
    Sophie Weil et al.
    Neuro-oncology, 19(10), 1316-1326 (2017-04-19)
    Primary and adaptive resistance against chemo- and radiotherapy and local recurrence after surgery limit the benefits from these standard treatments in glioma patients. Recently we found that glioma cells can extend ultra-long membrane protrusions, "tumor microtubes" (TMs), for brain invasion
    View All Related Papers

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