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WGA3

Sigma-Aldrich

GenomePlex® WGA Reamplification Kit

Reamplification of WGA product with minimal bias

Synonym(s):

GenomePlex® whole genome amplification reamplification kit, Whole genome reamplification kit

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

Pricing and availability is not currently available.

Quality Level

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

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clone

RM324, monoclonal, recombinant monoclonal

clone

SP13, monoclonal

clone

RM344, monoclonal, recombinant monoclonal

clone

RM314, monoclonal, recombinant monoclonal

species reactivity

rat, mouse

species reactivity

human (tested)

species reactivity

human

species reactivity

human

antibody form

purified immunoglobulin

antibody form

tissue culture supernatant

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Gene Information

mouse ... Calb2(12308)

Gene Information

human ... CALB2(794)

Gene Information

human ... CD3E(916)

Gene Information

human ... CD5(921)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

isotype

IgG

isotype

IgG

isotype

IgG

isotype

IgG

General description

GenomePlex® WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It allows the user to obtain even higher yields of desired DNA without disturbing the original, limited source of genomic DNA. The genomic DNA can be reamplified up to five cycles with minimal allele dropout producing an even higher yield. It provides the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Application

GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP.[1][2][3] It has also been used in the reamplification of WGA2-amplified genomic DNA to generate >10 μg gDNA for microarray hybridization.[4]

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit Components Only

Product No.
Description

  • Deoxynucleotide Mix, 10 mM

Kit Components Also Available Separately

Product No.
Description
SDS

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mL

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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