CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

This antibody recognizes the Cdt1.

Epitope: C-terminal half

Recombinant protein including amino acids 238-546 of human CDT1.

An independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000).

Detect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

Evaluated by western blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.

65 kDa

Unpurified rabbit serum containing 0.05% NaN3.

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

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