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1.03858

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GranuCult® plus Eosin Methylene Blue Agar

acc. HOLT-HARRIS and TEAGUE; For the isolation and differentiation of E. coli and other gram-negative bacteria

Synonym(s):

EMB agar, Holt-Harris & Teague (HHT) EMB agar, Eosin Methylene Blue Lactose Sucrose agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74
Pricing and availability is not currently available.
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Quality Level

form

powder

storage condition

dry media

technique(s)

microbiological culture: suitable

pH

7.1±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

15-25°C

suitability

selective for coliforms: Citrobacter spp.
selective for enterobacteriaceae (Genus: Proteus Vulgaris)

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P05500001511000Y0001045
manufacturer/tradename

BP

manufacturer/tradename

EDQM

manufacturer/tradename

USP

manufacturer/tradename

EDQM

grade

pharmaceutical primary standard

grade

pharmaceutical primary standard

grade

pharmaceutical primary standard

grade

pharmaceutical primary standard

form

solid

form

-

form

-

form

-

format

neat

format

neat

format

neat

format

neat

application(s)

pharmaceutical
pharmaceutical small molecule

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

General description

GranuCult® plus EMB Agar acc. HOLT-HARRIS and TEAGUE is a low selective and differential medium containing lactose and sucrose and two indicator dyes, Eosin and Methylene Blue. The use of these two indicators allows differentiation between colonies of lactose and/or sucrose fermenting and non-fermenting organisms. Sucrose is included since certain members of the coliform group of microorganism ferment sucrose more readily than lactose. This formulation does not discriminate between carbohydrate utilization (lactose or sucrose).
The peptones provide nitrogen, minerals and amino acids, lactose and sucrose provide the carbon sources. Di-potassium phosphate acts as a buffer whilst Eosin yellowish (Eosin Y) and methylene blue serve as differential indicators and inhibitors. Agar is the solidifying agent.
Coliforms produce blue-black colonies due to the incorporation of an eosin-methylene blue dye complex when the pH drops down. Colonies of Escherichia coli may show a characteristic green metallic luster due to the rapid fermentation of lactose. Lactose- and sucrose-non fermenting bacteria (like most Salmonella and Shigella) will form colourless or transparent amber-coloured colonies, but some strains of Salmonella and Shigella may not grow on this culture medium. Some Gram-positive bacteria and yeasts may grow on this culture medium and usually form pinpoint colonies.

Application

GranuCult® plus Eosin Methylene Blue Agar is a low selective, differential agar used to identify coliforms especially E. coli. It differentiates lactose fermenters from non-lactose fermenters.It is often the media used when testing the potability of water via the MPN method

Features and Benefits

  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to- batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allowing many supplements to be included, with no need to add these separately

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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Low-molecular-weight xylanase from Trichoderma viride
Ujiie, M.
Applied and Environmental Microbiology, 57, 1860-1862 (1994)
Saleh Sabiha-Hanim et al.
Bioresource technology, 102(2), 1234-1239 (2010-08-28)
Oil palm (Elaeis guineensis Jacq.) is one of the most important commercial crops for the production of palm oil, which generates 10.88 tons of oil palm fronds per hectare of plantation as a by-product. In this study, oil palm frond
Ichiro Kamei et al.
Bioresource technology, 126, 137-141 (2012-10-18)
We propose a new process of unified aerobic delignification and anaerobic saccharification and fermentation of wood by a single microorganism, the white-rot fungus Phlebia sp. MG-60. This fungus is able to selectively degrade lignin under aerobic solid state fermentation conditions
Maija Tenkanen et al.
The FEBS journal, 280(1), 285-301 (2012-11-22)
A minor xylanase, named XYN IV, was purified from the cellulolytic system of the fungus Trichoderma reesei Rut C30. The enzyme was discovered on the basis of its ability to attack aldotetraohexenuronic acid (HexA-2Xyl-4Xyl-4Xyl, HexA(3)Xyl(3)), releasing the reducing-end xylose residue.
J Gerasimova et al.
Mikrobiologiia, 81(4), 457-463 (2012-11-20)
Thermophilic strain JK1 was isolated from compost using xylan as a single carbon source. On the basis of 16S rRNA gene phylogenetic analysis and spo0A gene sequence similarity analysis, strain JK1 was identified as Geobacillus thermodenitrificans strain. During the exponential

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