5310-OP
HEPES
>= 99%, Free Acid, fine powder, OmniPur® Grade
Synonym(s):
HEPES, N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
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About This Item
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein/REAXYS Number:
883043
MDL number:
UNSPSC Code:
12352207
NACRES:
NA.21
Pricing and availability is not currently available.
Recommended Products
Product Name
HEPES, OmniPur® Grade, >= 99%, Free Acid
Quality Level
form
fine powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
useful pH range
6.8-8.2
pKa (25 °C)
7.5
shipped in
ambient
storage temp.
10-30°C
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1 of 4
This Item | MABN540 | SAB4501323 | SAB5200718 |
|---|---|---|---|
| Gene Information human ... GRM5(2915) | Gene Information human ... GRM5(2915) | Gene Information human ... GRM5(2915) | Gene Information rat ... Grm5(24418) |
| species reactivity human, rat | species reactivity human, mouse, rat | species reactivity mouse, rat, human | species reactivity mouse, human, rat |
| clone 11D9.1, monoclonal | clone N75/33, monoclonal | clone polyclonal | clone S75-33, monoclonal |
| biological source mouse | biological source mouse | biological source rabbit | biological source mouse |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
General description
Molecular Biology grade
A260 (0.1 M, water): ≤0.05 AU
A280 (0.1 M, water): ≤0.05 AU
Assay (dried basis): ≥99
DNase: None detected
Heavy metals (as Pb): ≤0.001%
Phosphatase: None detected
pKa at 25°C: 6.80-8.20
Protease: None detected
RNase: None detected
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
Application
<ul>
<li><strong>Phenothiazine appended thiophene derivative: a trilateral approach to copper ion detection in living cells and aqueous samples.</strong>: This study explores the use of HEPES buffer in the detection of copper ions, showcasing its utility in maintaining the stability of the sensor in aqueous solutions and live cell environments. The findings indicate that HEPES buffer plays a crucial role in the accuracy and efficiency of ion detection assays, which is essential for biochemists working with metal ion sensors (Ilakiyalakshmi et al., 2024).</li>
<li><strong>Evaluation of acute intravenous toxicity of HEPES: Is Good′s buffer good and safe enough for clinical utilization in nuclear medicine </strong>: This research assesses the safety of HEPES buffer for intravenous use, specifically its acute toxicity. The results are vital for biochemists in nuclear medicine, indicating that HEPES is a safe and effective buffering agent for clinical applications (Guleria et al., 2024).</li>
<li><strong>Nanoscale engineering of gold nanostars for enhanced photoacoustic imaging.</strong>: The study highlights the use of HEPES buffer in the synthesis of gold nanostars, which significantly improves photoacoustic imaging. This demonstrates the buffer′s importance in nanobiotechnology, particularly in enhancing the stability and functionality of nanomaterials used in imaging techniques (Zhang et al., 2024).</li>
<li><strong>Influx of zwitterionic buffer after intracytoplasmic sperm injection (ICSI) membrane piercing alters the transcriptome of human oocytes.</strong>: This article discusses how HEPES buffer influences the transcriptome of human oocytes post-ICSI, highlighting its role in reproductive biology and assisted reproductive technologies. The findings are crucial for biochemists focusing on fertility treatments and genetic studies (Mendola et al., 2024).</li>
<li><strong>One Method to Label Them All": A Single Fully Automated Protocol for GMP-Compliant 68Ga Radiolabeling of PSMA-11, Transposable to PSMA-I and PSMA-617.</strong>: This paper outlines a protocol using HEPES buffer for radiolabeling in nuclear medicine, emphasizing its application in producing radiopharmaceuticals. The research supports the buffer′s critical role in maintaining the quality and compliance of radiolabeling processes (Fouillet et al., 2024).</li>
</ul>
<li><strong>Phenothiazine appended thiophene derivative: a trilateral approach to copper ion detection in living cells and aqueous samples.</strong>: This study explores the use of HEPES buffer in the detection of copper ions, showcasing its utility in maintaining the stability of the sensor in aqueous solutions and live cell environments. The findings indicate that HEPES buffer plays a crucial role in the accuracy and efficiency of ion detection assays, which is essential for biochemists working with metal ion sensors (Ilakiyalakshmi et al., 2024).</li>
<li><strong>Evaluation of acute intravenous toxicity of HEPES: Is Good′s buffer good and safe enough for clinical utilization in nuclear medicine </strong>: This research assesses the safety of HEPES buffer for intravenous use, specifically its acute toxicity. The results are vital for biochemists in nuclear medicine, indicating that HEPES is a safe and effective buffering agent for clinical applications (Guleria et al., 2024).</li>
<li><strong>Nanoscale engineering of gold nanostars for enhanced photoacoustic imaging.</strong>: The study highlights the use of HEPES buffer in the synthesis of gold nanostars, which significantly improves photoacoustic imaging. This demonstrates the buffer′s importance in nanobiotechnology, particularly in enhancing the stability and functionality of nanomaterials used in imaging techniques (Zhang et al., 2024).</li>
<li><strong>Influx of zwitterionic buffer after intracytoplasmic sperm injection (ICSI) membrane piercing alters the transcriptome of human oocytes.</strong>: This article discusses how HEPES buffer influences the transcriptome of human oocytes post-ICSI, highlighting its role in reproductive biology and assisted reproductive technologies. The findings are crucial for biochemists focusing on fertility treatments and genetic studies (Mendola et al., 2024).</li>
<li><strong>One Method to Label Them All": A Single Fully Automated Protocol for GMP-Compliant 68Ga Radiolabeling of PSMA-11, Transposable to PSMA-I and PSMA-617.</strong>: This paper outlines a protocol using HEPES buffer for radiolabeling in nuclear medicine, emphasizing its application in producing radiopharmaceuticals. The research supports the buffer′s critical role in maintaining the quality and compliance of radiolabeling processes (Fouillet et al., 2024).</li>
</ul>
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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P Kielczynski et al.
IEEE transactions on ultrasonics, ferroelectrics, and frequency control, 46(1), 233-238 (2008-02-02)
In this paper the theoretical analysis and the results of testing of a piezoelectric cantilever for the investigation of material surfaces are presented. The cantilever consists of a thin piezoelectric plate bonded with a thin metal (e.g., molybdenum) foil. The
Charles F Baker et al.
Dalton transactions (Cambridge, England : 2003), (9)(9), 1298-1303 (2004-07-15)
New molybdenum(VI) nitride oxides were synthesised by the reaction of strontium nitride and calcium nitride with molybdenum foil at high temperature in sealed stainless steel crucibles. The reactions yielded single crystalline products determined by X-ray diffraction to form complex structures
J G Watkins et al.
The Review of scientific instruments, 79(10), 10F125-10F125 (2008-12-03)
Two modular arrays of Langmuir probes designed to handle a heat flux of up to 25 MW/m(2) for 10 s exposures have been installed in the lower divertor target plates of the DIII-D tokamak. The 20 pyrolytic graphite probe tips
Michael N Evans
Rapid communications in mass spectrometry : RCM, 22(14), 2211-2219 (2008-06-11)
A reactor for converting cellulose into carbon monoxide for subsequent oxygen isotopic analysis via continuous flow isotope ratio mass spectrometry is described. The system employs an induction heater to produce temperatures >or=1500 degrees C within a molybdenum foil crucible positioned
Dattatray J Late et al.
Nanotechnology, 19(26), 265605-265605 (2008-07-02)
Nanocrystalline lanthanum hexaboride (LaB(6)) films have been deposited on molybdenum foil by the pulsed laser deposition (PLD) technique. The as-deposited films were characterized by x-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The XRD pattern shows
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