Alpha-2B adrenergic receptor (UniProt: P18089; also known as Alpha-2 adrenergic receptor subtype C2, Alpha-2B adrenoreceptor, Alpha-2B adrenoceptor, Alpha-2BAR) is encoded by the ADRA2B (also known as ADRA2L1, ADRA2RL1) gene (Gene ID: 151) in human. Alpha-2-adrenergic receptors mediate part of the diverse biological effects of the endogenous catecholamines epinephrine and norepinephrine. Three subtypes of alpha-2-adrenergic receptors, 2A, 2B, and 2C, have been identified in multiple species. Alpha-2B adrenoreceptor is a heptahelical membrane protein of the G-protein coupled receptor 1 family. The extracellular regions of transmembrane (TM) segments 3, 5, and 6 are believed to be involved in ligand binding and intracellular ends of TM3 and TM6 participate in G-protein coupling. A deletion variant of the ADRA2B gene has been linked to the emotional enhancement of memory. This deletion variant is associated with greater extracellular nor-epinephrine availability and it predicts greater capacity for emotionally enhanced memory and increased tendency for intrusive traumatic memory. Mutations in ADRA2B gene have been linked to familial adult myoclonic epilepsy that is characterized by cortical myoclonus and variable occurrence of epileptic seizures.

This polyclonal antibody detects alpha-2B adrenergic receptor in MCF-10A cells. It targets an epitope within 14 amino acids from the internal region.

KLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of human alpha-2B adrenergic receptor.

Anti-Alpha 2b Adrenergic Receptor, Cat. No. ABN1481, is a highly specific rabbit polyclonal antibody that targets Alpha-2B adrenergic receptor and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected Alpha 2b Adrenergic Receptor in human cerebellum, human cerebral cortex, rat cerebellum, and rat hippocampus tissues.

Research Category
Neuroscience

Evaluated by Western Blotting in MCF-10A cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Alpha 2b Adrenergic Receptor in 10 µg of MCF-10A cell lysate.

~52 kDa observed; 49.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.