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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human (immunogen homology)
technique(s)
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ANG(283)
General description
Protein fat-free homolog (Fat Free), also known as Vacuolar protein sorting-associated protein 51 homolog and Another new gene 2 protein (ANG2). Fat Free is a component of the Golgi-associated retrograde protein (GARP) complex which mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). The GARP complex maintains protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. Fat Free participates in acid hydrolase receptor transport, likely by promoting tethering and fusion of endosome-derived carriers to the TGN.
Immunogen
KLH-conjugated linear peptide corresponding to human Fat Free.
Application
Anti-Fat Free Antibody is a highly specific rabbit polyclonal antibody, that targets Fat free & has been tested in western blotting.
Research Category
Signaling
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Fat Free in 15 µg of mouse brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Fat Free in 15 µg of mouse brain tissue lysate.
Target description
~80 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~73 kDa and ~86 kDa
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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