FCMAB317PE
Milli-Mark® Anti-NeuN-PE Antibody, clone A60
clone A60, Milli-Mark®, from mouse
Synonym(s):
Neuron-Specific Nuclear Protein, Neuna60, A60
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
PE
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A60, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG1
Related Categories
General description
NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5.
Specificity
Antibody recognizes Neuronal Nuclei.
Immunogen
Purified cell nuclei from mouse brain.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
This Milli-Mark Anti-NeuN-PE Antibody, clone A60 is validated for use in FC for the detection of NeuN.
Quality
Evaluated by flow cytometry using U251 cells.
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
U251 cells
U251 cells
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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