biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4C12.1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG2b
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... HGF(3082)
General description
Anti-HGF (Scatter Factor) (Ab-1), mouse monoclonal, clone 4C12.1, recognizes the ~82 kDa hepatocyte growth factor in macrophages, endothelial & Kupffer cells. It is validated for use in WB & IP.
Purified mouse monoclonal antibody. Recognizes the ~82 kDa HGF protein.
Recognizes the ~82 kDa hepatocyte growth factor protein in macrophages, endothelial, and Kupffer cells.
Immunogen
Human
recombinant, human HGF protein
Application
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Immunoprecipitation (10 µl/mg protein lysate)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Reconstitution
Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Analysis Note
Positive Control
Endothelial cells, Kupffer cells, or macrophages
Endothelial cells, Kupffer cells, or macrophages
Other Notes
Antibody should be titrated for optimal results in individual systems.
Toi, M., et al. 1998. Clin. Cancer Res.4, 659.
Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp.212, 133.
Birchmeier, C., et al. 1997. Ciba Found. Symp.212, 16.
Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol.12, 537.
Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp.212, 198.
Rosen, E.M., et al. 1997. Ciba Found. Symp.212, 215.
Vande Woude, G.F., et al. 1997. Ciba Found Symp.212, 119.
Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp.212, 133.
Birchmeier, C., et al. 1997. Ciba Found. Symp.212, 16.
Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol.12, 537.
Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp.212, 198.
Rosen, E.M., et al. 1997. Ciba Found. Symp.212, 215.
Vande Woude, G.F., et al. 1997. Ciba Found Symp.212, 119.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service