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GR01L

Sigma-Aldrich

Anti-EGFR (Ab-1) Mouse mAb (528)

lyophilized, clone 528, Calbiochem®

Synonym(s):

Anti-Epidermal Growth Factor Receptor

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

528, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

should not react with

rat, mouse

manufacturer/tradename

Calbiochem®

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Anti-EGFR (Ab-1) Mouse mAb (528) lyophilized, clone 528, Calbiochem®

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Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) lyophilized, clone EGFR.1, Calbiochem®

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Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

Anti-EGFR (Ab-1) Mouse mAb (528) liquid, clone 528, Calbiochem®

GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) liquid, clone EGFR.1, Calbiochem®

GR15

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

clone

528, monoclonal

clone

EGFR.1, monoclonal

clone

528, monoclonal

clone

EGFR.1, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

isotype

IgG2a

isotype

IgG2b

isotype

IgG2a

isotype

IgG2b

General description

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Recognizes the EGF receptor protein in A431 cells and normal skin.

Immunogen

partially purified, human EGFR from human A431 cells

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Analysis Note

Positive Control
A-431 cells or skin tissue

Other Notes

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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