Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), andorthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.

Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein.

Epitope: transactivation domain

Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of human LEF-1 and mouse myeloma cells.

Research Category
Stem Cell Research

Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells

This Anti-LEF-1 Antibody, transactivation domain, clone 3A12 is validated for use in WB for the detection of LEF-1.

Western blot

Optimal working dilutions must be determined by end user.

Replaces: 04-1130

Format: Purified

Purified immunoglobulin. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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