Tartrate-resistant acid phosphatase type 5 (TRAcP), also referred to as Acp5, is secreted by osteoclasts during bone resorption. Release of TRAcP was stimulated by resorbogenic cytokines and repressed by calcitonin (a resorption inhibitor). TRAcP is also known to be expressed in high quantities in active macrophages and dendritic cells and play an essential part in presenting antigens to T cells. Inactive TRAcP released from adipose tissue macrophages has been shown to bring on hyperplastic obesity, keeping lipid metabolism and insulin sensitivity normal.

Full length purified protein corresponding to human TRAcP.

Anti-TRAcP Antibody, clone 9C5 detects level of TRAcP & has been published & validated for use in IH, IP, DB, ELISA, IC & WB.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

Dot Blot Analysis: A representative lot was used by an independent laboratory in DB. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Janckila, A.J., et al. (1996). The Journal of Histochemistry and Cytochemistry. 44(3):233-244.)

Evaluated by Immunohistochemistry in large malignant B-cells in hairy cell leukemia tissue.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected TRAcP in large malignant B-cells in hairy cell leukemia tissue.

36 kDa calculated

Format: Purified

Protein G

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Large malignant B-cells in hairy cell leukemia tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.