Sodium channel protein type 2 subunit alpha (UniProt Q99250; also known as HBSC II, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.2) is encoded by the SCN2A (also known as EIEE11, BFIS3, NAC2, SCN2A1, SCN2A2) gene (Gene ID 6326) in human. Sodium channels are integral plasma membrane proteins that conduct Na+ transport across the plasma membrane. They are classified as either voltage-gated or ligand-gated based on the nature of the triggering mechanism for channel activation. Voltage-gated sodium channels (VGSCs) are also called voltage-sensitive, voltage-dependent, or Nav channels. VGSCs normally consist of an alpha subunit that forms the ion-conducting pore and one to two beta subunits that have several functions including channel gating modulation, although the expression of the alpha subunit alone is sufficient to produce a functional channel. There exist nine known alpha subunits (Nav1.1 through Nav1.5 encoded by SCN1A through SCN5A and Nav1.6 through Nav1.9 encoded by SCN8A through SCN11A) and four beta subunits (Navbeta1 through Navbeta4 encoded by SCN1B through SCN4B). Beta 1 and beta 3 interact with the alpha subunit non-covalently, whereas beta 2 and beta 4 associate with alpha via disulfide bond. Nav1.2 is a multi-pass membrane protein with 24 membrane regions, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-124 & 1777-2005).

Clone 5H10.2 targets an epitope in the C-terminal cytoplasmic domain.

Epitope: C-terminal cytoplasmic domain.

GST-tagged recombinant human SCN2A C-terminal cytoplasmic domain fragment.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-SCN2A Antibody, clone 5H10.2 is validated for use in Western Blotting for the detection of SCN2A.

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SCN2A in 10 µg of human cortex tissue lysate.

~227-275 kDa observed. 228.0 kDa calculated. The braod banding pattern observed is consistent with the detection of differentially glycosylated and phosphorylated Nav1.2 protein. Uncharacterized band(s) may appear in some lysates.

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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