Neurofascin is a ankyrin binding protein that may be involved in myelination, neurite extension, synaptogenesis, axonal guidance and neuron-glial cell interactions. There are two known isoforms, NF155 and NF186. NF155 is found in oligodendrocytes, and is involved in neurite outgrowth and neuronal adhesion. NF186 is expressed in neurons, and may optimize communication between neurons by anchoring the axon initial segment (AIS).

Other Homologies: Human (99% sequence homology).

Recombinant protein corresponding to rat Pan-Neurofascin.

Detect Neurofascin using this mouse monoclonal antibody, Anti-Pan-Neurofascin Antibody, clone L11A/41 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pan-Neuorfascin in rat pons tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pan-Neurofascin in 10 µg of rat brain tissue lysate.

~185 kDa and ~155 kDa observed. Three isoforms are known to exist at 138 kDa, 134 kDa, and 132 kDa due to alternative splicing. This protein has been observed at ~186 kDa, ~180 kDa, ~166 kDa and ~155 kDa (Kriebel, M., et al. (2012). Int J Biochem Cell Biol. 44(5):694-697.). This protein may undergo post-translational modification.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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