biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3F1, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HTT(3064)
General description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.
Recognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
This Anti-HTT Mouse mAb (3F1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of HTT.
Immunogen
A recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
U-2 OS cells, HeLa NE cells, Human salivary gland tissue
U-2 OS cells, HeLa NE cells, Human salivary gland tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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