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About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
material
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
2 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
volume
2 mL
working volume
≤2 mL
2 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
General description
Amicon® Ultra-2 centrifugal filter devices offer fast ultrafiltration. It has the potential to recover high concentration factors and easy concentrate from dilute and complex sample matrices. Amicon® Ultra-2 devices are supplied non-sterile and are for single use only.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Amicon® Ultra-2 Centrifugal Filter Unit has been used:
- to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
- Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
- Desalting, buffer exchange, or diafiltration
Features and Benefits
- Amicon® Ultra 2ml 10K 24PK
- Concentrates 2 mL down to 15-70 μl
- 98% sample recovery
- Fast processing time - 10 to 30 minute spin times
- Engineered dead stop
- The reverse spin capability provides consistent recoveries
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
Linkage
Replaces: 4205
Other Notes
The Amicon® Ultra-2 device is supplied with two tubes. During operation, one tube is used to collect filtrate; the other to cap the device during concentration and subsequently to recover the concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research use only and not for use in diagnostic procedures.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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