24-0940

Pepsin from porcine gastric mucosa

CP

• Synonym(s): Pepsin A, Pepsin from hog stomach
• CAS Number: 9001-75-6
• EC Number: 3.4.23.1 (BRENDA, IUBMB)
• MDL number: MFCD00081840
• UNSPSC Code: 41115710
• grade: CP

Properties

• grade: CP
• form: powder
• mol wt: 35 kDa
• availability: available only in Japan
• dilution: (for analytical testing)
• UniProt accession no.: P00791
• Gene Information: pig ... LOC396892(396892)

Description

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P284 - P304 + P340 - P305 + P351 + P338 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable