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MilliporeSigma

472476

Sigma-Aldrich

Chloroform

contains amylenes as stabilizer, ACS reagent, ≥99.8%

Synonym(s):

Methylidyne trichloride, Trichloromethane

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About This Item

Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein/REAXYS Number:
1731042
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.04

grade:
ACS reagent
assay:
≥99.8%
bp:
60.5-61.5 °C (lit.)
vapor pressure:
160 mmHg ( 20 °C)
Pricing and availability is not currently available.

grade

ACS reagent

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.8%

form

liquid

contains

amylenes as stabilizer

impurities

Acetone and aldehyde, passes test
Acid and chloride, passes test
Free chlorine (Cl), passes test
≤0.03% (water)

evapn. residue

0.001%

color

APHA: ≤10

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1 of 4

This Item
480150319988372978
grade

ACS reagent

grade

ACS reagent

grade

ACS reagent

grade

anhydrous

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

impurities

Acetone and aldehyde, passes test, Free chlorine (Cl), passes test, Acid and chloride, passes test, ≤0.03% (water)

impurities

Acetone and aldehyde, passes test, Acid and chloride, passes test, Free chorine (Cl), passes test, <0.03% water

impurities

Acetone and aldehyde, passes test, Acid and chloride, passes test, Free chlorine (Cl), passes test, H2SO4, passes test

impurities

<0.001% water, 0.005% water (100mL)

Application

Chloroform was used in the process for isolation of RNA from yeast.[1]
It may be used in the following processes:
  • Synthesis of colloidal hydrophobic CdSe core nanocrystals (quantum dots).[2]
  • Preparing phospholipid solutions.[3]
  • As a solvent in the photolysis reaction of ruthenium complexes.[4]
  • Solvent for recrystallization.[5]
  • Reference compound to detect the presence of chloroform in tobacco smokes.[6]
  • Extractant in solvent extraction process.[7]

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash


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A Simple RNA Preparation Procedure from Yeast for Northern Blot Using Hot Phenol.
Wei Y.
Bio-protocol, 2(12) (2012)
Kinetics and Mechanism of the Photooxidation of Bis (bipyridine) dichlororuthenium (II) and the Photoreduction of Bis (bipyridine) dichlororuthenium (III) in Chloroform.
Sathiyabalan S and Hoggard PE.
Inorganic Chemistry, 34(18), 4562-4571 (1995)
The affinity of two antimicrobial peptides derived from bovine milk proteins for model lipid membranes.
Barzyk W, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 343(1), 104-110 (2009)
Systematic studies on the breakdown of p,p'-DDT in tobacco smokes. Presence of methyl chloride, dichloromethane, and chloroform in tobacco smokes.
Chopra NM and Sherman LR.
Analytical Chemistry, 44(6), 1036-1038 (1972)
Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots.
Sukhanova A, et al.
Protocol Exchange (2012)

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